Hyaluronic Acid
Due to its ability to hydrate and plump the skin, hyaluronic acid is commonly used in skincare products and cosmetic treatments to moisturize the skin, reduce the appearance of wrinkles and fine lines, and improve overall skin texture and elasticity.
It is also used in medical applications, such as in joint injections for the treatment of osteoarthritis and as a dermal filler in cosmetic procedures.
Ongoing viscosity measurement of hyaluronic acid ensures consistency and quality control during the production. By monitoring viscosity, manufacturers can maintain desired product characteristics and ensure batch-to-batch consistency.
RPV systems shown below are suitable for measuring hyaluronic acid.
Selected filters:
Hyaluronic acid - EU.Ph. Monograph
Different manufacturing processes and conditions can result in variations in molecular weight and viscosity, which can impact the performance of the final product. By monitoring viscosity, manufacturers can maintain desired product characteristics and ensure product consistency.
Viscosity measurements also help in formulating hyaluronic acid-based products for specific applications. The viscosity of HA solutions or gels can affect their flow properties, spreadability, and ease of application. By understanding the viscosity profile of different formulations, formulators can tailor products to meet specific requirements. These include viscosity for topical creams or gels, or injectability for medical applications.
Viscosity is often correlated with other properties of hyaluronic acid solutions, such as their ability to retain moisture, lubricate, or provide cushioning. By measuring viscosity, researchers and manufacturers can predict the performance of HA-based products in various applications, such as skincare, ophthalmology, orthopedics, and wound healing.
In medical applications, such as intra-articular injections for osteoarthritis or ophthalmic surgeries, the viscosity of hyaluronic acid solutions is critical for ensuring proper delivery and effectiveness. Measuring viscosity helps ensure that the HA solution has the appropriate rheological properties for the intended use, such as optimal flow through syringes or controlled spreading in tissues.
Viscosity measurements also play a role in research and development efforts aimed at understanding the properties and behaviour of hyaluronic acid under different conditions. Researchers may investigate factors such as temperature, concentration, and molecular weight to optimize HA formulations for specific applications.
What solvents do I need ?
Prior to viscosity measurement, hyaluronic acid is dissolved in a so called buffer solution. This is a phosphate buffer solution with sodium chloride added. After the measurement, the sample path is cleaned with buffer solution followed by a drying sequence using acetone.
What is the temperature of measurement?
Hyaluronic Acid is measured at 25 degrees C. The solution is charged into a calibrated glass capillary viscometer. The viscometer is fitted in a constant temperature viscometer bath. Temperature stability of the PSL Rheotek RPV-3 bath is +/- 0.005 degrees C, when it is correctly installed and operated.
What are the installation requirements ?
A laboratory environment with stable temperature. The PSL Rheotek Viscometer for Hyaluronic Acid does not need to be installed in a fume hood
How long does it take?
A complete measurement cycle takes approximately one hour. This includes cleaning and drying of the sample path.
Can I measure Hyaluronic Acid in an easier way?
GPC/SEC is commonly used for determining the molecular weight of polymers and macromolecules.